Cocoyam, an important staple crop in Ghana, provides edible leafy vegetable and starchy cormels. Due to difficulty in getting primers for cocoyam, sweetpotato EST SSR primers were used to amplify cocoyam lines. Genomic DNA was isolated from 10 sweetpotato and nine cocoyam cultivars. Ten sweetpotato accessions were screened alongside three cocoyam cultivars, using 22 EST Sweetpotato SSR primers, 13 of which could amplify cocoyam sequences and were subsequently used to screen nine cocoyam cultivars. Thirteen random primers were also used for diversity study.